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antigen retrieval  (Vector Laboratories)


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    Structured Review

    Vector Laboratories antigen retrieval
    Antigen Retrieval, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unmasking+solution/Antigen+Unmasking+Solution%2C+Citric+Acid+Based/pmc13112402-75-7-9
    Average 96 stars, based on 2600 article reviews
    antigen retrieval - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: Porphyromonas gingivalis-derived outer membrane vesicles travel from oral to distant organs via blood circulation in a time-dependent manner.
    Article Snippet: Masson’s trichrome staining and a commercial TRAP staining kit (Sigma, 387A-1KT) were employed accordingly. .. For immunohistochemical (IHC) staining, antigen retrieval was performed using a specific unmasking solution (Vector Laboratories Inc., Burlingame, CA), followed by permeabilization with 0.2% Triton X-100. .. After blocking with normal serum, endogenous peroxidase activity was suppressed by treating the sections with BLOXALL reagent (Vector Laboratories Inc., Burlingame, CA).

    Article Title: Porphyromonas gingivalis- derived outer membrane vesicles travel from oral to distant organs via blood circulation in a time-dependent manner
    Article Snippet: Masson’s trichrome staining and a commercial TRAP staining kit (Sigma, 387A-1KT) were employed accordingly. .. For immunohistochemical (IHC) staining, antigen retrieval was performed using a specific unmasking solution (Vector Laboratories Inc., Burlingame, CA), followed by permeabilization with 0.2% Triton X-100. .. After blocking with normal serum, endogenous peroxidase activity was suppressed by treating the sections with BLOXALL reagent (Vector Laboratories Inc., Burlingame, CA).

    Immunohistochemistry:

    Article Title: Porphyromonas gingivalis-derived outer membrane vesicles travel from oral to distant organs via blood circulation in a time-dependent manner.
    Article Snippet: Masson’s trichrome staining and a commercial TRAP staining kit (Sigma, 387A-1KT) were employed accordingly. .. For immunohistochemical (IHC) staining, antigen retrieval was performed using a specific unmasking solution (Vector Laboratories Inc., Burlingame, CA), followed by permeabilization with 0.2% Triton X-100. .. After blocking with normal serum, endogenous peroxidase activity was suppressed by treating the sections with BLOXALL reagent (Vector Laboratories Inc., Burlingame, CA).

    Article Title: Porphyromonas gingivalis- derived outer membrane vesicles travel from oral to distant organs via blood circulation in a time-dependent manner
    Article Snippet: Masson’s trichrome staining and a commercial TRAP staining kit (Sigma, 387A-1KT) were employed accordingly. .. For immunohistochemical (IHC) staining, antigen retrieval was performed using a specific unmasking solution (Vector Laboratories Inc., Burlingame, CA), followed by permeabilization with 0.2% Triton X-100. .. After blocking with normal serum, endogenous peroxidase activity was suppressed by treating the sections with BLOXALL reagent (Vector Laboratories Inc., Burlingame, CA).

    Staining:

    Article Title: Porphyromonas gingivalis-derived outer membrane vesicles travel from oral to distant organs via blood circulation in a time-dependent manner.
    Article Snippet: Masson’s trichrome staining and a commercial TRAP staining kit (Sigma, 387A-1KT) were employed accordingly. .. For immunohistochemical (IHC) staining, antigen retrieval was performed using a specific unmasking solution (Vector Laboratories Inc., Burlingame, CA), followed by permeabilization with 0.2% Triton X-100. .. After blocking with normal serum, endogenous peroxidase activity was suppressed by treating the sections with BLOXALL reagent (Vector Laboratories Inc., Burlingame, CA).

    Article Title: Porphyromonas gingivalis- derived outer membrane vesicles travel from oral to distant organs via blood circulation in a time-dependent manner
    Article Snippet: Masson’s trichrome staining and a commercial TRAP staining kit (Sigma, 387A-1KT) were employed accordingly. .. For immunohistochemical (IHC) staining, antigen retrieval was performed using a specific unmasking solution (Vector Laboratories Inc., Burlingame, CA), followed by permeabilization with 0.2% Triton X-100. .. After blocking with normal serum, endogenous peroxidase activity was suppressed by treating the sections with BLOXALL reagent (Vector Laboratories Inc., Burlingame, CA).

    Incubation:

    Article Title: Gene regulatory dynamics during craniofacial development in a carnivorous marsupial
    Article Snippet: .. Frontal head sections (7 μm thick) on superfrost slides (Platinum Pro, Grale) were deparaffinized and rehydrated according to standard methods , followed by antigen retrieval using pH 8.8 unmasking solution (Vector) at 99°C for 30 min. We then incubated the sections with either rabbit anti-H3K4me3 primary antibody (1:500 dilution; Abcam ab8580) or rabbit anti-H3K27ac primary antibody (1:500, Abcam ab4729). .. Sections were then incubated with Alexa Fluor 555 donkey anti-rabbit antibody (1:500 dilution; Abcam in 10% horse serum in PBS with 0.1% Triton X-100; Sigma).

    Article Title: Induction of activating transcription factor 3 (ATF3) in the cerebral cortex of a mouse model of blast-induced traumatic brain injury
    Article Snippet: .. Paraffin Sects. (5 μm-thick) were deparaffinized with xylene and rehydrated with ethanol, then immersed in unmasking solution (H-3300; Vector Laboratories, Newark, CA) for antigenic retrieval and heated in an autoclave (121°C) for 5 min. After incubated with blocking reagent (Nacalai Tesque, Kyoto, Japan), sections were incubated with primary antibodies at 4 °C overnight. .. The primary antibodies were rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (BS-0199R; Thermo Fisher), goat polyclonal anti-Iba1 (011–27991, Wako, Osaka, Japan), mouse monoclonal anti-neuronal nuclei (NeuN) (MAB377; Millipore, Burlington, MA), or rabbit monoclonal anti-ATF3 (ab305293; Abcam, Cambridge, UK) antibodies.

    Article Title: Induction of activating transcription factor 3 (ATF3) in the cerebral cortex of a mouse model of blast-induced traumatic brain injury.
    Article Snippet: .. Paraffin Sects. (5 μm-thick) were deparaffinized with xylene and rehydrated with ethanol, then immersed in unmasking solution (H-3300; Vector Laboratories, Newark, CA) for antigenic retrieval and heated in an autoclave (121°C) for 5 min. After incubated with blocking reagent (Nacalai Tesque, Kyoto, Japan), sections were incubated with primary antibodies at 4 °C overnight. .. The primary antibodies were rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (BS-0199R; Thermo Fisher), goat polyclonal anti-Iba1 (011–27991, Wako, Osaka, Japan), mouse monoclonal anti-neuronal nuclei (NeuN) (MAB377; Millipore, Burlington, MA), or rabbit monoclonal anti-ATF3 (ab305293; Abcam, Cambridge, UK) antibodies.

    Blocking Assay:

    Article Title: Induction of activating transcription factor 3 (ATF3) in the cerebral cortex of a mouse model of blast-induced traumatic brain injury
    Article Snippet: .. Paraffin Sects. (5 μm-thick) were deparaffinized with xylene and rehydrated with ethanol, then immersed in unmasking solution (H-3300; Vector Laboratories, Newark, CA) for antigenic retrieval and heated in an autoclave (121°C) for 5 min. After incubated with blocking reagent (Nacalai Tesque, Kyoto, Japan), sections were incubated with primary antibodies at 4 °C overnight. .. The primary antibodies were rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (BS-0199R; Thermo Fisher), goat polyclonal anti-Iba1 (011–27991, Wako, Osaka, Japan), mouse monoclonal anti-neuronal nuclei (NeuN) (MAB377; Millipore, Burlington, MA), or rabbit monoclonal anti-ATF3 (ab305293; Abcam, Cambridge, UK) antibodies.

    Article Title: Induction of activating transcription factor 3 (ATF3) in the cerebral cortex of a mouse model of blast-induced traumatic brain injury.
    Article Snippet: .. Paraffin Sects. (5 μm-thick) were deparaffinized with xylene and rehydrated with ethanol, then immersed in unmasking solution (H-3300; Vector Laboratories, Newark, CA) for antigenic retrieval and heated in an autoclave (121°C) for 5 min. After incubated with blocking reagent (Nacalai Tesque, Kyoto, Japan), sections were incubated with primary antibodies at 4 °C overnight. .. The primary antibodies were rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (BS-0199R; Thermo Fisher), goat polyclonal anti-Iba1 (011–27991, Wako, Osaka, Japan), mouse monoclonal anti-neuronal nuclei (NeuN) (MAB377; Millipore, Burlington, MA), or rabbit monoclonal anti-ATF3 (ab305293; Abcam, Cambridge, UK) antibodies.



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